Embolon, Thessaloniki, had been defined regarding the function of reproduction and you can ploidy
BOWEN, S.T., DURKIN, J.P., STERLING, Grams. and you will CLARK, L.S. (1978) Artemia hemoglobins: hereditary version from inside the parthenogenetic and zygogenetic communities. Bull., 155, 273287.
Cysts out-of parthenogenetic Artemia strains accumulated on Citros and you may Yards. Embolon saltworks for the Northern Greece was evaluated due to their possible have fun with from inside the aquaculture. Next characterizations was basically performed: tumefaction and you can naupliar biometrics, tumor hatching properties, oily acidic character of your own nauplii, caloric posts away from nauplii held on twenty five° C plus a fridge (4 8° C). The above mentioned investigations demonstrates the two Artemia strains studied display a services to be used from inside the aquaculture, particularly in culturing fresh-drinking water species. The brand new biometrical studies out-of cysts, nauplii and you will people shows a leading degree of similarity together with other parthenogenetic stresses off certain geographical offer online religious chat room, but particularly that have tetraploid Artemia away from The country of spain. The brand new Greek Artemia stresses can not be thought to be ‘offer to possess aquacultural uses except if best management of new saltworks try hoping.
Because the brine shrimp Artemia are out of high monetary importance, it is essential to analyse the brand new ‘industrial services of various communities with regard to the possibility software inside the aquaculture. During the early in the day knowledge, a couple North Greek Artemia populations used in Citros, Piera and Meters. These people were found to be parthenogenetic and you may tetraploid (Abatzopoulos et al., 1986). Research of studies to your DNA reassociation Kinetics as well as on 13 enzymatic systems of these two populations indicate that it fall into one species (Abatzopoulos ainsi que al., 1987).
Biol
Contained in this report we summary of the newest biometrical features away from cysts, nauplii and you may adults, for the hatching properties regarding cysts, we.e. hatching rates, hatching payment, hatching performance and you may hatching efficiency, on the changes in inactive weight and you can caloric articles from instar-We nauplii managed on room temperature and cold storage criteria, and on brand new fatty acid users away from instar-I nauplii.
The 2 communities out-of Northern Greek Artemia evaluated come from the fresh new saltworks off Citros, Pieria (C filter systems), and regarding the saltworks of M. Embolon, Thessaloniki (Myself strain). Cysts was amassed when you look at the 1983, processed based on Sorgeloos mais aussi al. (1978), and you can kept in sealed plastic handbags at -25° C, to own upcoming explore.
Mature brine shrimps was in fact anesthezied inside the chlorofrom-saturated and you will measured not as much as an excellent dissection microscope playing with good Leitz micrometer, Next measurements were did : full duration, abdominal length, maximum width out of brood wallet, depth from third abdomianal phase, thickness out-of lead, length of first antenna, maximum diameter of state-of-the-art vision, and you may length anywhere between complex sight (Amat, 1980). Specifications was performed towards the dogs – test proportions = 50 dogs-originating from wild cysts and you will cultured around simple labouratory standards (fungus as the restaurants, temperatures off 25 + 1° C, and you may seawater away from thirty-five% salinity). The details was indeed mathematically managed by data out of covariance (Sokal Rohlf, 1981).
Hatching analyses have been carried out predicated on Vanhaecke Sorgeloos (1983). Hatching abilities, hatching percentage, hatching speed and hatching returns was analysed pursuing the rates and you may hatching output were analysed pursuing the Sorgeloos et al. (1978), Bruggeman et al. (1980), Vanhaecke Sorgeloos (1982), and you may Vanhaecke Sorgeloos (1983), correspondingly.
This new hatching of one’s cysts in addition to break up and you may line of the new nauplii (instar-l so you can instar-III) were accomplished according to process demonstrated inside Vanhaecke elizabeth al. (1983).
The latest types of nauplii was indeed dehydrated in the an incubator in the 55°C getting forty eight many hours. Each naupliar phase learnt, 4 to 6 duplicated trials, around ten milligrams lifeless material was basically burned totally into the absolute oxygen in addition to their caloric blogs try mentioned for the a great Phillipson microbomb calorimeter.
In advance of studies, samples had been homogenized during the an enthusiastic ultrasonic homogenizer (Sonifier B12) and overall lipids was removed according to types of Bilgh Dyer (1959). Saponification and methylation had been performed since the described because of the Shauer Simpson (1978). Fatty acidic methylesters was indeed inserted when you look at the good capillary line (twenty-five m bonded silica; inner diameter; 0.thirty two mm; liquide stage : SILAR 10C, film thickness: 0.3um) installed in the a beneficial Carlo Erba Super 2350 gasoline chromatograph.